Vitamin B12

Vitamin B12

Origin
: China
CAS Number
: 68-19-9
HS Code
: 293626
Basic Info
Physical State
: Solid
Appearance / Color
: Dark red to reddish powder
Odor
: Odorless
Moisture Content (%)
: <5%
Synonyms & Trade Names
: Cyanocobalamin; Cobalamin; Vitamin B12 1%
Total Plate Count (TPC)
: <50,000 CFU/g
Yeast & Mould
: <500 CFU/g
E. Coli
: Negative/25g
Coliform Bacteria
: <100 CFU/g
Salmonella
: Negative/25g
Shelf Life
: 24 months
Packaging Size
: 1 kg
Packaging Type
: Aluminum foil bag / brown glass bottle
Storage Conditions
: Cool, dark, dry; 2-8°C; protect from light
Halal Certification
: Available
Kosher Certification
: Available
ISO / HACCP
: Yes
Food Grade / Regulatory Status
: Feed/Pharmaceutical grade
Total Volatile Basic Nitrogen (TVN/TVBN, mg/100g)
: Not applicable
Active Ingredient Assay (%)
: 1% min cyanocobalamin
Antioxidant Treatment
: None
Species of Origin Declaration
: N/A (fermentation)
EU Feed Additive Authorization No.
: E880
Aflatoxin B1
: Not applicable
Heavy Metals — Feed (As, Pb, Hg, Cd)
: Compliant
Dioxins & PCBs (pg WHO-TEQ/g fat)
: Not applicable
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Technical Document

Brief Overview
Cyanocobalamin or well-known as synthetically produced Vitamin B12 is an additive in animal feed to overcome the deficiency of vitamin B12 in animals with pernicious anemia and other conditions. Methylcobalamin and adenosylcobalamin are used in an animal body through the conversion of cyanocobalamin. Cyanocobalamin crystalline is powdery within the dark red color.
Manufacturing Process
The present invention relates to a process for producing vitamin B12 comprises the steps of culturing a strain of the genus Propionibacterium in a first fermenter under anaerobic conditions to obtain a culture of Propionibacterium, transferring at least part of the culture obtained in the previous step to a second fermenter and subjecting this culture to oxygen, replacing in the first fermenter part of the volume transferred in the previous step with fresh culture medium and repeating all the steps at least once.